Journal: mAbs

Article Title: Generation of robust bispecific antibodies through fusion of single-domain antibodies on IgG scaffolds: a comprehensive comparison of formats

doi: 10.1080/19420862.2023.2189432

Figure Lengend Snippet: Antibody binding to PD-L1 and HER2 using FIDA. (a) Assessment of bispecific binding functionality through changes in apparent Rh in response to addition of fluorescent PD-L1-DY490 ± unlabeled HER2 antigens in solution (b) Assessment of bispecific binding functionality through changes in apparent Rh in response to addition of fluorescent HER2-DY490 ± unlabeled PD-L1 antigens in solution. (c) Antibody titration curves investigating binding in mono- and bispecific binding environments. The binding curves were generated by titrating antibody against the fluorescently labeled primary antigen ± an unlabeled secondary antigen. Since the secondary antigen does not carry any fluorescence, it can only affect the signal indirectly through complexation with the bsAb, which is in itself binding the primary antigen. This is to test if the titration curves change in response to addition of the unlabeled secondary antigen. The coloring scheme is the same as in (a) and (b).

Article Snippet: Rh values were obtained by Taylorgrams to FIDA Software v2.3 (Fida Biosystems) with a Taylorgram fraction of 75%.

Techniques: Binding Assay, Titration, Generated, Labeling, Fluorescence

Journal: mAbs

Article Title: Generation of robust bispecific antibodies through fusion of single-domain antibodies on IgG scaffolds: a comprehensive comparison of formats

doi: 10.1080/19420862.2023.2189432

Figure Lengend Snippet: Affinity binding constants and the related goodness-of-fit from antibody titration curves using FIDA. The titration curves were generated by titrating antibodies against a fluorescently labeled antigen ± the unlabeled antigen. The goodness-of-fit is evaluated using R 2 and root mean squared error (RMSE). Affinity values in brackets marked with an asterisk indicate affinity values previously reported elsewhere. 20 , 21 , 33 , 34 .

Article Snippet: Rh values were obtained by Taylorgrams to FIDA Software v2.3 (Fida Biosystems) with a Taylorgram fraction of 75%.

Techniques: Binding Assay, Titration, Generated, Labeling